LOCUS       NC_001489               7478 bp ss-RNA     linear   VRL 08-DEC-2008
DEFINITION  Hepatitis A virus, complete genome.
ACCESSION   NC_001489
VERSION     NC_001489.1  GI:9626732
DBLINK      BioProject: PRJNA15308
KEYWORDS    RefSeq.
SOURCE      Hepatitis A virus
  ORGANISM  Hepatitis A virus
            Viruses; ssRNA positive-strand viruses, no DNA stage;
            Picornavirales; Picornaviridae; Hepatovirus.
REFERENCE   1  (bases 1 to 7478)
  AUTHORS   Cohen,J.I., Ticehurst,J.R., Purcell,R.H., Buckler-White,A. and
            Baroudy,B.M.
  TITLE     Complete nucleotide sequence of wild-type hepatitis A virus:
            comparison with different strains of hepatitis A virus and other
            picornaviruses
  JOURNAL   J. Virol. 61 (1), 50-59 (1987)
   PUBMED   3023706
REFERENCE   2  (bases 1 to 7478)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2004) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from M14707.
            There are polypyrimidine tracts at positions 98 to 137 and 712 to
            720.  This sequence supersedes several previously published partial
            sequences of the hepatitis A virus wild type virus.  These partial
            genomic sequences are found under accession numbers K00386, M14114
            and M14115.
            Draft entry and computer-readable copy of sequence in [1] kindly
            provided by J.I.Cohen, 05-AUG-1987.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7478
                     /organism="Hepatitis A virus"
                     /mol_type="genomic RNA"
                     /db_xref="taxon:12092"
     gene            1..7478
                     /locus_tag="HAVgs1"
                     /db_xref="GeneID:1493917"
     misc_RNA        1..7478
                     /locus_tag="HAVgs1"
                     /note="hepatitis A viral genome"
                     /db_xref="GeneID:1493917"
     gene            735..7418
                     /locus_tag="HAVgp1"
                     /db_xref="GeneID:1493918"
     CDS             735..7418
                     /locus_tag="HAVgp1"
                     /note="polyprotein a (alt.)"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="NP_041007.1"
                     /db_xref="GI:9626733"
                     /db_xref="GeneID:1493918"
                     /translation="MNMSRQGIFQTVGSGLDHILSLADIEEEQMIQSVDRTAVTGASY
                     FTSVDQSSVHTAEVGSHQVEPLRTSVDKPGSKKTQGEKFFLIHSADWLTTHALFHEVA
                     KLDVVKLLYNEQFAVQGLLRYHTYARFGIEIQVQINPTPFQQGGLICAMVPGDQSYGS
                     IASLTVYPHGLLNCNINNVVRIKVPFIYTRGAYHFKDPQYPVWELTIRVWSELNIGTG
                     TSAYTSLNVLARFTDLELHGLTPLSTQMMRNEFRVSTTENVVNLSNYEDARAKMSFAL
                     DQEDWKSDPSQGGGIKITHFTTWTSIPTLAAQFPFNASDSVGQQIKVIPVDPYFFQMT
                     NTNPDQKCITALASICQMFCFWRGDLVFDFQVFPTKYHSGRLLFCFVPGNELIDVSGI
                     TLKQATTAPCAVMDITGVQSTLRFRVPWISDTPYRVNRYTKSAHQKGEYTAIGKLIVY
                     CYNRLTSPSNVASHVRVNVYLSAINLECFAPLYHAMDVTTQVGDDSGGFSTTVSTEQN
                     VPDPQVGITTMKDLKGKANRGKMDVSGVQAPVGAITTIEDPVLAKKVPETFPELKPGE
                     SRHTSDHMSIYKFMGRSHFLCTFTFNSNNKEYTFPITLSSTSNPPHGLPSTLRWFFNL
                     FQLYRGPLDLTIIITGATDVDGMAWFTPVGLAVDTPWVEKESALSIDYKTALGAVRFN
                     TRRTGNIQIRLPWYSYLYAVSGALDGLGDKTDSTFGLVSIQIANYNHSDEYLSFSCYL
                     SVTEQSEFYFPRAPLNSNAMLSTESMMSRIAAGDLESSVDDPRSEEDKRFESHIECRK
                     PYKELRLEVGKQRLKYAQEELSNEVLPPPRKMKGLFSQAKISLFYTEEHEIMKFSWRG
                     VTADTRALRRFGFSLAAGRSVWTLEMDAGVLTGRLIRLNDEKWTEMKDDKIVSLIEKF
                     TSNKYWSKVNFPHGMLDLEEIAANSKDFPNMSETDLCFLLHWLNPKKINLADRMLGLS
                     GVQEIKEQGVGLIAECRTFLDSIAGTLKSMMFGFHHSVTVEIINTVLCFVKSGILLYV
                     IQQLNQDEHSHIIGLLRVMNYADIGCSVISCGKVFSKMLETVFNWQMDSRMMELRTQS
                     FSNWLRDICSGITIFKNFKDAIYWLYTKLKDFYEVNYGKKKDILNILKDNQQKIEKAI
                     EEADEFCILQIQDVEKFEQYQKGVDLIQKLRTVHSMAQVDPNLMVHLSPLRDCIARVH
                     QKLKNLGSINQAMVTRCEPVVCYLYGKRGGGKSLTSIALATKICKHYGVEPEKNIYTK
                     PVASDYWDGYSGQLVCIIDDIGQNTTDEDWSDFCQLVSGCPMRLNMASLEEKGRHFSS
                     PFIIATSNWSNPSPKTVYVKEAIDRRLHFKVEVKPASFFKNPHNDMLNVNLAKTNDAI
                     KDMSCVDLIMDGHNVSLMDLLSSLVMTVEIRKQNMTEFMELWSQGISDDDNDSAVAEF
                     FQSFPSGEPSNSKLSGFFQSVTNHKWVAVGAAVGILGVLVGGWFVYKHFSRKEEEPIP
                     AEGVYHGVTKPKQVIKLDADPVESQSTLEIAGLVRKNLVQFGVGEKNGCVRWVMNALG
                     VKDDWLLVPSHAYKFEKDYEMMEFYFNRGGTYYSISAGNVVIQSLDVGFQDVVLMKVP
                     TIPKFRDITQHFIKKGDVPRALNRLATLVTTVNGTPMLISEGPLKMEEKATYVHKKND
                     GTTVDLTVDQAWRGKGEGLPGMCGGALVSSNQSIQNAILGIHVAGGNSILVAKLVTQE
                     MFQNIDKKIESQRIMKVEFTQCSMNVVSKTLFRKSPIYHHIDKTMINFPAAMPFSKAE
                     IDPMAVMLSKYSLPIVEEPEDYKEASIFYQNKIVGKTQLVDDFLDLDMAITGAPGIDA
                     INMDSSPGFPYVQEKLTKRDLIWLDENGLLLGVHPRLAQRILFNTVMMENCSDLDVVF
                     TTCPKDELRPLEKVLESKTRAIDACPLDYSILCRMYWGPAISYFHLNPGFHTGVAIGI
                     DPDRQWDELFKTMIRFGDVGLDLDFSAFDASLSPFMIREAGRIMSELSGTPSHFGTAL
                     INTIIYSKHLLYNCCYHVCGSMPSGSPCTALLNSIINNVNLYYVFSKIFGKSPVFFCQ
                     ALKILCYGDDVLIVFSRDVQIDNLDLIGQKIVDEFKKLGMTATSADKNVPQLKPVSEL
                     TFLKRSFNLVEDRIRPAISEKTIWSLIAWQRSNAEFEQNLENAQWFAFMHGYEFYQKF
                     YYFVQSCLEKEMIEYRLKSYDWWRMRFYDQCFICDLS"
     mat_peptide     735..803
                     /locus_tag="HAVgp1"
                     /product="1A VP4a mature peptide (alt.)"
                     /protein_id="NP_740548.1"
                     /db_xref="GI:25121593"
     misc_feature    981..1463
                     /locus_tag="HAVgp1"
                     /note="Picornavirus capsid protein domain_like.
                     Picornaviruses are non-enveloped plus-strand ssRNA animal
                     viruses with icosahedral capsids composed of 60 copies
                     each of 4 virus encoded proteins; alignment includes
                     picornaviridae, like poliovirus, hepatitis A...; Region:
                     rhv_like; cd00205"
                     /db_xref="CDD:119412"
     misc_feature    order(1050..1052,1125..1127,1131..1133,1137..1139,
                     1185..1187,1242..1244,1338..1340,1419..1421)
                     /locus_tag="HAVgp1"
                     /note="drug-binding pocket [chemical binding]; other site"
                     /db_xref="CDD:119412"
     misc_feature    1632..2177
                     /locus_tag="HAVgp1"
                     /note="Picornavirus capsid protein domain_like.
                     Picornaviruses are non-enveloped plus-strand ssRNA animal
                     viruses with icosahedral capsids composed of 60 copies
                     each of 4 virus encoded proteins; alignment includes
                     picornaviridae, like poliovirus, hepatitis A...; Region:
                     rhv_like; cd00205"
                     /db_xref="CDD:119412"
     misc_feature    order(1716..1718,1812..1814,1818..1820,1824..1826,
                     1872..1874,1941..1943,2013..2015,2064..2066,2145..2147)
                     /locus_tag="HAVgp1"
                     /note="drug-binding pocket [chemical binding]; other site"
                     /db_xref="CDD:119412"
     misc_feature    2469..2999
                     /locus_tag="HAVgp1"
                     /note="Picornavirus capsid protein domain_like.
                     Picornaviruses are non-enveloped plus-strand ssRNA animal
                     viruses with icosahedral capsids composed of 60 copies
                     each of 4 virus encoded proteins; alignment includes
                     picornaviridae, like poliovirus, hepatitis A...; Region:
                     rhv_like; cl13999"
                     /db_xref="CDD:265416"
     misc_feature    order(2550..2552,2628..2630,2634..2636,2640..2642,
                     2682..2684,2769..2771,2838..2840,2883..2885,2952..2954)
                     /locus_tag="HAVgp1"
                     /note="drug-binding pocket [chemical binding]; other site"
                     /db_xref="CDD:119412"
     misc_feature    2934..3245
                     /locus_tag="HAVgp1"
                     /note="Protein of unknown function (DUF3840); Region:
                     DUF3840; pfam12944"
                     /db_xref="CDD:193417"
     misc_feature    4410..4718
                     /locus_tag="HAVgp1"
                     /note="RNA helicase; Region: RNA_helicase; pfam00910"
                     /db_xref="CDD:250218"
     misc_feature    5313..5876
                     /locus_tag="HAVgp1"
                     /note="3C cysteine protease (picornain 3C); Region:
                     Peptidase_C3; pfam00548"
                     /db_xref="CDD:249949"
     misc_feature    6423..7220
                     /locus_tag="HAVgp1"
                     /note="RNA_dep_RNAP: RNA-dependent RNA polymerase (RdRp)
                     is an essential protein encoded in the genomes of all RNA
                     containing viruses with no DNA stage. RdRp catalyzes
                     synthesis of the RNA strand complementary to a given RNA
                     template. RdRps of many viruses are...; Region:
                     RNA_dep_RNAP; cd01699"
                     /db_xref="CDD:238843"
     misc_feature    order(6678..6695,6852..6857,6975..6977,6981..6986,
                     7137..7142)
                     /locus_tag="HAVgp1"
                     /note="active site"
                     /db_xref="CDD:238843"
     misc_feature    order(6678..6683,6981..6986)
                     /locus_tag="HAVgp1"
                     /note="metal ion binding site [ion binding]; other site"
                     /db_xref="CDD:238843"
     misc_feature    6855..6857
                     /locus_tag="HAVgp1"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]; other site"
                     /db_xref="CDD:238843"
     gene            741..7418
                     /locus_tag="HAVgp2"
                     /db_xref="GeneID:1493919"
     CDS             741..7418
                     /locus_tag="HAVgp2"
                     /note="polyprotein b (alt.)"
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="NP_041008.1"
                     /db_xref="GI:9626734"
                     /db_xref="GeneID:1493919"
                     /translation="MSRQGIFQTVGSGLDHILSLADIEEEQMIQSVDRTAVTGASYFT
                     SVDQSSVHTAEVGSHQVEPLRTSVDKPGSKKTQGEKFFLIHSADWLTTHALFHEVAKL
                     DVVKLLYNEQFAVQGLLRYHTYARFGIEIQVQINPTPFQQGGLICAMVPGDQSYGSIA
                     SLTVYPHGLLNCNINNVVRIKVPFIYTRGAYHFKDPQYPVWELTIRVWSELNIGTGTS
                     AYTSLNVLARFTDLELHGLTPLSTQMMRNEFRVSTTENVVNLSNYEDARAKMSFALDQ
                     EDWKSDPSQGGGIKITHFTTWTSIPTLAAQFPFNASDSVGQQIKVIPVDPYFFQMTNT
                     NPDQKCITALASICQMFCFWRGDLVFDFQVFPTKYHSGRLLFCFVPGNELIDVSGITL
                     KQATTAPCAVMDITGVQSTLRFRVPWISDTPYRVNRYTKSAHQKGEYTAIGKLIVYCY
                     NRLTSPSNVASHVRVNVYLSAINLECFAPLYHAMDVTTQVGDDSGGFSTTVSTEQNVP
                     DPQVGITTMKDLKGKANRGKMDVSGVQAPVGAITTIEDPVLAKKVPETFPELKPGESR
                     HTSDHMSIYKFMGRSHFLCTFTFNSNNKEYTFPITLSSTSNPPHGLPSTLRWFFNLFQ
                     LYRGPLDLTIIITGATDVDGMAWFTPVGLAVDTPWVEKESALSIDYKTALGAVRFNTR
                     RTGNIQIRLPWYSYLYAVSGALDGLGDKTDSTFGLVSIQIANYNHSDEYLSFSCYLSV
                     TEQSEFYFPRAPLNSNAMLSTESMMSRIAAGDLESSVDDPRSEEDKRFESHIECRKPY
                     KELRLEVGKQRLKYAQEELSNEVLPPPRKMKGLFSQAKISLFYTEEHEIMKFSWRGVT
                     ADTRALRRFGFSLAAGRSVWTLEMDAGVLTGRLIRLNDEKWTEMKDDKIVSLIEKFTS
                     NKYWSKVNFPHGMLDLEEIAANSKDFPNMSETDLCFLLHWLNPKKINLADRMLGLSGV
                     QEIKEQGVGLIAECRTFLDSIAGTLKSMMFGFHHSVTVEIINTVLCFVKSGILLYVIQ
                     QLNQDEHSHIIGLLRVMNYADIGCSVISCGKVFSKMLETVFNWQMDSRMMELRTQSFS
                     NWLRDICSGITIFKNFKDAIYWLYTKLKDFYEVNYGKKKDILNILKDNQQKIEKAIEE
                     ADEFCILQIQDVEKFEQYQKGVDLIQKLRTVHSMAQVDPNLMVHLSPLRDCIARVHQK
                     LKNLGSINQAMVTRCEPVVCYLYGKRGGGKSLTSIALATKICKHYGVEPEKNIYTKPV
                     ASDYWDGYSGQLVCIIDDIGQNTTDEDWSDFCQLVSGCPMRLNMASLEEKGRHFSSPF
                     IIATSNWSNPSPKTVYVKEAIDRRLHFKVEVKPASFFKNPHNDMLNVNLAKTNDAIKD
                     MSCVDLIMDGHNVSLMDLLSSLVMTVEIRKQNMTEFMELWSQGISDDDNDSAVAEFFQ
                     SFPSGEPSNSKLSGFFQSVTNHKWVAVGAAVGILGVLVGGWFVYKHFSRKEEEPIPAE
                     GVYHGVTKPKQVIKLDADPVESQSTLEIAGLVRKNLVQFGVGEKNGCVRWVMNALGVK
                     DDWLLVPSHAYKFEKDYEMMEFYFNRGGTYYSISAGNVVIQSLDVGFQDVVLMKVPTI
                     PKFRDITQHFIKKGDVPRALNRLATLVTTVNGTPMLISEGPLKMEEKATYVHKKNDGT
                     TVDLTVDQAWRGKGEGLPGMCGGALVSSNQSIQNAILGIHVAGGNSILVAKLVTQEMF
                     QNIDKKIESQRIMKVEFTQCSMNVVSKTLFRKSPIYHHIDKTMINFPAAMPFSKAEID
                     PMAVMLSKYSLPIVEEPEDYKEASIFYQNKIVGKTQLVDDFLDLDMAITGAPGIDAIN
                     MDSSPGFPYVQEKLTKRDLIWLDENGLLLGVHPRLAQRILFNTVMMENCSDLDVVFTT
                     CPKDELRPLEKVLESKTRAIDACPLDYSILCRMYWGPAISYFHLNPGFHTGVAIGIDP
                     DRQWDELFKTMIRFGDVGLDLDFSAFDASLSPFMIREAGRIMSELSGTPSHFGTALIN
                     TIIYSKHLLYNCCYHVCGSMPSGSPCTALLNSIINNVNLYYVFSKIFGKSPVFFCQAL
                     KILCYGDDVLIVFSRDVQIDNLDLIGQKIVDEFKKLGMTATSADKNVPQLKPVSELTF
                     LKRSFNLVEDRIRPAISEKTIWSLIAWQRSNAEFEQNLENAQWFAFMHGYEFYQKFYY
                     FVQSCLEKEMIEYRLKSYDWWRMRFYDQCFICDLS"
     mat_peptide     741..803
                     /locus_tag="HAVgp2"
                     /product="1A VP4b mature peptide (alt.)"
                     /protein_id="NP_740549.1"
                     /db_xref="GI:25121594"
     mat_peptide     804..1469
                     /locus_tag="HAVgp2"
                     /product="1B VP2 mature peptide"
                     /protein_id="NP_740550.1"
                     /db_xref="GI:25121595"
     misc_feature    981..1463
                     /locus_tag="HAVgp2"
                     /note="Picornavirus capsid protein domain_like.
                     Picornaviruses are non-enveloped plus-strand ssRNA animal
                     viruses with icosahedral capsids composed of 60 copies
                     each of 4 virus encoded proteins; alignment includes
                     picornaviridae, like poliovirus, hepatitis A...; Region:
                     rhv_like; cd00205"
                     /db_xref="CDD:119412"
     misc_feature    order(1050..1052,1125..1127,1131..1133,1137..1139,
                     1185..1187,1242..1244,1338..1340,1419..1421)
                     /locus_tag="HAVgp2"
                     /note="drug-binding pocket [chemical binding]; other site"
                     /db_xref="CDD:119412"
     mat_peptide     1470..2207
                     /locus_tag="HAVgp2"
                     /product="1C VP3 mature peptide"
                     /protein_id="NP_740551.1"
                     /db_xref="GI:25121596"
     misc_feature    1632..2177
                     /locus_tag="HAVgp2"
                     /note="Picornavirus capsid protein domain_like.
                     Picornaviruses are non-enveloped plus-strand ssRNA animal
                     viruses with icosahedral capsids composed of 60 copies
                     each of 4 virus encoded proteins; alignment includes
                     picornaviridae, like poliovirus, hepatitis A...; Region:
                     rhv_like; cd00205"
                     /db_xref="CDD:119412"
     misc_feature    order(1716..1718,1812..1814,1818..1820,1824..1826,
                     1872..1874,1941..1943,2013..2015,2064..2066,2145..2147)
                     /locus_tag="HAVgp2"
                     /note="drug-binding pocket [chemical binding]; other site"
                     /db_xref="CDD:119412"
     mat_peptide     2208..3107
                     /locus_tag="HAVgp2"
                     /product="1D VP1 mature peptide"
                     /protein_id="NP_740552.1"
                     /db_xref="GI:25121597"
     misc_feature    2469..2999
                     /locus_tag="HAVgp2"
                     /note="Picornavirus capsid protein domain_like.
                     Picornaviruses are non-enveloped plus-strand ssRNA animal
                     viruses with icosahedral capsids composed of 60 copies
                     each of 4 virus encoded proteins; alignment includes
                     picornaviridae, like poliovirus, hepatitis A...; Region:
                     rhv_like; cl13999"
                     /db_xref="CDD:265416"
     misc_feature    order(2550..2552,2628..2630,2634..2636,2640..2642,
                     2682..2684,2769..2771,2838..2840,2883..2885,2952..2954)
                     /locus_tag="HAVgp2"
                     /note="drug-binding pocket [chemical binding]; other site"
                     /db_xref="CDD:119412"
     misc_feature    2934..3245
                     /locus_tag="HAVgp2"
                     /note="Protein of unknown function (DUF3840); Region:
                     DUF3840; pfam12944"
                     /db_xref="CDD:193417"
     mat_peptide     3108..3674
                     /locus_tag="HAVgp2"
                     /product="2A mature peptide"
                     /note="Capsid assembly signal 2A is removed from the
                     VP1-2A protein by a postassembly proteolytic event that
                     presumably involves a host proteinase such as FXa; capsid
                     assembly signal 2A"
                     /protein_id="NP_740553.1"
                     /db_xref="GI:25121598"
     mat_peptide     3675..3995
                     /locus_tag="HAVgp2"
                     /product="2B mature peptide"
                     /protein_id="NP_740554.1"
                     /db_xref="GI:25121599"
     mat_peptide     3996..5000
                     /locus_tag="HAVgp2"
                     /product="2C mature peptide"
                     /protein_id="NP_740555.1"
                     /db_xref="GI:25121600"
     misc_feature    4410..4718
                     /locus_tag="HAVgp2"
                     /note="RNA helicase; Region: RNA_helicase; pfam00910"
                     /db_xref="CDD:250218"
     mat_peptide     5001..5222
                     /locus_tag="HAVgp2"
                     /product="3A mature peptide"
                     /protein_id="NP_740556.1"
                     /db_xref="GI:25121601"
     mat_peptide     5223..5291
                     /locus_tag="HAVgp2"
                     /product="3B (VPg) mature peptide"
                     /protein_id="NP_740557.1"
                     /db_xref="GI:25121602"
     mat_peptide     5292..5948
                     /locus_tag="HAVgp2"
                     /product="3C mature peptide"
                     /protein_id="NP_740558.1"
                     /db_xref="GI:25121603"
     misc_feature    5313..5876
                     /locus_tag="HAVgp2"
                     /note="3C cysteine protease (picornain 3C); Region:
                     Peptidase_C3; pfam00548"
                     /db_xref="CDD:249949"
     mat_peptide     5949..7415
                     /locus_tag="HAVgp2"
                     /product="3D mature peptide"
                     /protein_id="NP_740559.1"
                     /db_xref="GI:25121604"
     misc_feature    6423..7220
                     /locus_tag="HAVgp2"
                     /note="RNA_dep_RNAP: RNA-dependent RNA polymerase (RdRp)
                     is an essential protein encoded in the genomes of all RNA
                     containing viruses with no DNA stage. RdRp catalyzes
                     synthesis of the RNA strand complementary to a given RNA
                     template. RdRps of many viruses are...; Region:
                     RNA_dep_RNAP; cd01699"
                     /db_xref="CDD:238843"
     misc_feature    order(6678..6695,6852..6857,6975..6977,6981..6986,
                     7137..7142)
                     /locus_tag="HAVgp2"
                     /note="active site"
                     /db_xref="CDD:238843"
     misc_feature    order(6678..6683,6981..6986)
                     /locus_tag="HAVgp2"
                     /note="metal ion binding site [ion binding]; other site"
                     /db_xref="CDD:238843"
     misc_feature    6855..6857
                     /locus_tag="HAVgp2"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]; other site"
                     /db_xref="CDD:238843"
     variation       871
                     /locus_tag="HAVgp2"
                     /note="c in pHAVlb39, t in pHAVlb113"
     variation       2196
                     /locus_tag="HAVgp2"
                     /note="g in pHAVlb39, a in pHAVlb1307"
     variation       6216
                     /locus_tag="HAVgp2"
                     /note="t in pHAVlb93, c in pHAVlb24"
ORIGIN      
        1 ttcaagaggg gtctccggga atttccggag tccctcttgg aagtccatgg tgaggggact
       61 tgatacctca ccgccgtttg cctaggctat aggctaaatt ttccctttcc cttttccctt
      121 tcctattccc tttgttttgc ttgtaaatat taattcctgc aggttcaggg ttcttaaatc
      181 tgtttctcta taagaacact catttttcac gctttctgtc ttctttcttc cagggctctc
      241 cccttgccct aggctctggc cgttgcgccc ggcggggtca actccatgat tagcatggag
      301 ctgtaggagt ctaaattggg gacacagatg tttggaacgt caccttgcag tgttaacttg
      361 gctttcatga atctctttga tcttccacaa ggggtaggct acgggtgaaa cctcttaggc
      421 taatacttct atgaagagat gccttggata gggtaacagc ggcggatatt ggtgagttgt
      481 taagacaaaa accattcaac gccggaggac tgactctcat ccagtggatg cattgagtgg
      541 attgactgtc agggctgtct ttaggcttaa ttccagacct ctctgtgctt agggcaaaca
      601 tcatttggcc ttaaatggga ttctgtgaga ggggatccct ccattgacag ctggactgtt
      661 ctttggggcc ttatgtggtg tttgcctctg aggtactcag gggcatttag gtttttcctc
      721 attcttaaat aataatgaac atgtctagac aaggtatttt ccagactgtt gggagtggtc
      781 ttgaccacat cctgtctttg gcagacattg aggaagagca aatgattcaa tcagttgata
      841 ggactgcagt gactggtgct tcttatttta cttctgtgga tcaatcttca gttcatacag
      901 ctgaggttgg atcacaccag gttgaacctt tgagaacctc tgttgataaa cccggttcaa
      961 agaagactca gggagagaaa tttttcttga ttcattctgc agattggctt actacacatg
     1021 ctcttttcca tgaagttgca aaattggatg tggtgaaatt attatacaat gagcagtttg
     1081 ctgttcaagg gttgttgaga taccatacat atgcaagatt tggcattgaa attcaagttc
     1141 agataaaccc tacacctttc caacaggggg gattgatctg tgctatggtt cctggtgacc
     1201 agagctatgg ttctatagca tcattgactg tttatcctca tggtttgtta aattgcaata
     1261 ttaacaatgt ggttagaata aaggttccat ttatttacac aagaggtgct taccacttta
     1321 aagatccaca atacccagtt tgggaattga caattagagt ttggtcagaa ttaaatattg
     1381 ggacaggaac ttcagcttat acttcactca atgttttagc tagatttaca gatttggagt
     1441 tgcatggatt aactcctctt tctacacaaa tgatgagaaa tgaatttagg gtcagtacta
     1501 ctgagaatgt ggtgaatctg tcaaattatg aagatgcaag agcaaagatg tcttttgctt
     1561 tggatcagga agattggaaa tctgatccgt cccagggtgg tgggatcaaa attactcatt
     1621 ttactacttg gacatctatt ccaactttgg ctgctcagtt tccatttaat gcttcagact
     1681 cagttggtca acaaattaaa gttattccag ttgacccata ttttttccaa atgacaaata
     1741 cgaatcctga ccaaaaatgt ataactgctt tggcttctat ttgtcagatg ttttgttttt
     1801 ggagaggaga tcttgtcttt gattttcaag tttttcccac caaatatcat tcaggtagat
     1861 tactgttttg ttttgttcct ggcaatgagc taatagatgt ttctggaatc acattaaagc
     1921 aagcaactac tgctccttgt gcagtaatgg atattacagg agtgcagtca actttgagat
     1981 ttcgtgttcc ctggatttct gacactcctt acagagtgaa caggtataca aagtcagcac
     2041 atcagaaagg tgagtacact gccattggga agcttattgt gtattgttat aacagattga
     2101 cctctccttc taacgttgct tcccatgtca gagtgaatgt ttatctttca gcaattaact
     2161 tggaatgttt tgctcctctt tatcatgcta tggatgttac tacacaagtt ggagatgatt
     2221 ctggaggttt ttcaacaaca gtttctacag aacagaatgt tccagatccc caagttggta
     2281 taacaaccat gaaagatttg aaaggaaaag ctaacagagg gaaaatggat gtttcaggag
     2341 tacaagcacc tgtgggagct atcacaacaa ttgaggatcc agttttagca aagaaagtac
     2401 ctgagacatt tcctgaattg aaacctggag aatccagaca tacatcagat catatgtcca
     2461 tctacaagtt tatgggaagg tctcatttct tgtgcacttt tacattcaat tcaaataata
     2521 aagagtacac atttcctata accttgtctt caacctctaa tcctcctcat ggtttgccat
     2581 caacactgag gtggtttttc aacttgtttc agttgtatag agggccttta gatctgacaa
     2641 ttattattac aggagcaact gatgtagatg gcatggcctg gttcactcca gtaggtcttg
     2701 ccgttgatac tccttgggta gagaaggagt cagctttgtc tattgactac aaaactgctc
     2761 ttggagctgt cagatttaac acaaggagaa cagggaacat tcagattaga ttaccatggt
     2821 attcttattt atatgctgtg tctggagcac tggatggttt gggtgacaag acagattcta
     2881 catttggatt ggtttctatt cagattgcaa attacaatca ttctgatgaa tacttgtctt
     2941 ttagttgtta tttgtctgtc acagaacaat cagagtttta ttttcccaga gctccattga
     3001 actcaaatgc catgttatcc actgaatcaa tgatgagcag aattgcagct ggagacttgg
     3061 agtcatcagt ggatgatcct agatcagagg aagataaaag atttgagagt catatagaat
     3121 gcaggaagcc atataaagaa ctgagattag aagttgggaa acaaagactc aagtatgctc
     3181 aggaagaatt gtcaaatgaa gtacttccac cccctaggaa aatgaaggga ctgttttcac
     3241 aagccaaaat ttctcttttt tatactgagg agcatgaaat aatgaagttt tcctggagag
     3301 gtgtgactgc tgatactaga gctttaagga ggtttggatt ctctttggcc gcaggcagaa
     3361 gtgtgtggac tcttgaaatg gatgctgggg ttcttactgg gagactgatt agattgaatg
     3421 atgagaaatg gacagaaatg aaggatgaca agattgtttc attgattgaa aagtttacaa
     3481 gtaacaaata ttggtccaaa gtgaatttcc cacatgggat gttggatctt gaagaaattg
     3541 ctgccaattc taaggatttt cctaacatgt ctgaaacgga tttgtgtttc ttgctgcatt
     3601 ggttaaatcc aaagaaaatt aatttagcag atagaatgct tggattgtct ggagttcagg
     3661 aaattaaaga acaaggtgtt ggattaatag cagagtgtag aactttctta gattctattg
     3721 ctggaacttt aaaatctatg atgtttggat ttcatcattc tgtgactgtt gaaattataa
     3781 acactgtgct ctgttttgtt aagagtggaa ttttgcttta tgtaatacaa caattgaatc
     3841 aggatgaaca ttctcacata attggtttgt tgagagtcat gaattatgca gatattggtt
     3901 gttcagttat ttcatgtggc aaagtttttt ccaaaatgct ggaaacagtc tttaattggc
     3961 aaatggactc cagaatgatg gagttaagga ctcagagttt ttccaactgg ttaagagata
     4021 tttgttctgg gatcaccatt tttaaaaact tcaaggatgc aatttattgg ctttatacaa
     4081 aattaaagga cttttatgaa gtgaattatg gcaagaagaa ggacatttta aatattctta
     4141 aagataacca acaaaaaata gagaaagcca ttgaggaagc cgatgaattt tgcattttgc
     4201 aaatccaaga tgtggaaaaa tttgaacagt atcagaaagg ggttgacttg atacaaaaat
     4261 tgagaactgt tcattcaatg gctcaggttg atccaaattt aatggttcat ttgtcacctt
     4321 tgagagattg tatagcaaga gttcatcaga aacttaaaaa ccttggatct ataaatcagg
     4381 caatggtaac gagatgtgag ccagttgttt gttatttata tggcaaaaga gggggaggaa
     4441 agagcttaac atcaattgca ttggcaacca aaatttgtaa acattatggt gttgagcctg
     4501 aaaagaatat ctatactaaa cctgtggctt cagattactg ggatggatat agtggacaat
     4561 tagtttgcat cattgatgat attggccaaa acacaacaga tgaggattgg tcagattttt
     4621 gtcagttagt gtcaggatgt ccaatgagat taaacatggc ctctcttgag gagaagggta
     4681 ggcatttttc ttctcctttt ataatagcaa cttcaaattg gtcaaatcca agtccaaaaa
     4741 cagtttatgt taaggaagca attgaccgca gactccattt caaggttgaa gttaaacctg
     4801 cttcattttt caaaaatcct cacaatgata tgttgaatgt taatttagct aaaacaaatg
     4861 atgcaatcaa agatatgtct tgtgttgatt tgataatgga tggacataat gtttcattga
     4921 tggatttgct cagttcttta gtcatgacag ttgaaattag aaaacaaaac atgactgaat
     4981 tcatggagtt gtggtctcag ggaatttcag atgatgataa tgatagtgca gtagctgagt
     5041 ttttccagtc ttttccatct ggtgaaccat cgaactctaa attatctggc tttttccaat
     5101 ctgttactaa tcacaagtgg gttgctgtgg gagctgcagt tggcattctt ggagtgctcg
     5161 ttggaggatg gtttgtgtat aagcatttct cccgcaaaga ggaggaacca atcccagctg
     5221 aaggggtata tcatggtgta actaagccca agcaagtgat taaattagat gcagatccag
     5281 tagaatctca gtcaactttg gaaatagcag gactggttag gaagaacttg gttcagtttg
     5341 gagttggaga gaagaatgga tgtgtgagat gggttatgaa tgccttggga gtgaaagatg
     5401 attggctgct tgtgccttcc catgcttata aatttgagaa agattatgaa atgatggagt
     5461 tttattttaa tagaggtgga acttactatt caatttcagc tggtaatgtt gttattcaat
     5521 ctttggatgt gggattccag gatgttgttc tgatgaaggt tcctacaatt cctaagttta
     5581 gagatattac tcagcatttt attaagaaag gggatgtgcc tagagctttg aatcgcctgg
     5641 caacattagt gacaactgta aatggaaccc ctatgttaat ttctgagggc ccactaaaga
     5701 tggaagagaa agctacttat gttcataaga aaaatgatgg tacaacagtt gatttaactg
     5761 tggatcaggc atggagagga aaaggcgaag gtcttcctgg aatgtgtggt ggggccttgg
     5821 tttcatcgaa tcaatctata cagaatgcaa tcttgggcat ccatgttgct ggaggaaatt
     5881 caattcttgt tgcaaaattg gttactcaag aaatgttcca aaatattgat aagaaaattg
     5941 aaagtcagag aattatgaaa gtggagttta ctcagtgttc aatgaatgtg gtctccaaaa
     6001 cgctttttag aaagagtccc atttatcatc acattgataa aaccatgatt aattttcctg
     6061 cagctatgcc cttttctaaa gctgaaattg atccaatggc tgtgatgtta tctaagtatt
     6121 cattacctat tgtagaagaa ccagaggatt ataaagaggc ttcaattttt tatcaaaata
     6181 aaatagtggg taagactcag ttagttgatg attttttaga tcttgatatg gccattacag
     6241 gggccccagg aattgatgct atcaacatgg attcatctcc tggatttcct tatgtccagg
     6301 agaagttgac caaaagagat ttaatttggt tggatgaaaa tggtttattg ctgggagttc
     6361 atccaagatt ggctcagaga atcttattca atactgtcat gatggaaaat tgttctgatt
     6421 tggatgttgt ttttacaacc tgtccaaaag atgaattgag accattagag aaagtgttgg
     6481 aatcaaaaac aagagctatt gatgcttgtc ctctggatta ctcaattttg tgccgaatgt
     6541 attggggtcc agctattagt tattttcatt tgaatccagg tttccataca ggtgttgcta
     6601 ttggcataga tcctgataga cagtgggatg aattatttaa aacaatgata agattcggag
     6661 atgttggtct tgatttagat ttctctgctt ttgatgctag tcttagtcca tttatgatta
     6721 gagaagcagg tagaatcatg agtgaactat ctggaactcc atcccatttt ggcacagctc
     6781 ttatcaatac tatcatttat tccaagcatt tgctgtataa ctgttgttac catgtctgtg
     6841 gttcaatgcc ctctgggtct ccttgtacag ctttgctaaa ttcaattatt aataatgtca
     6901 atttgtatta tgtgttttcc aagatatttg gaaagtctcc agttttcttt tgtcaggctt
     6961 tgaagattct ctgttatgga gatgatgttt taatagtttt ctctcgagat gttcagattg
     7021 ataatcttga tttgattgga caaaaaattg tagatgagtt taagaaactt ggcatgacag
     7081 ctacttctgc tgacaagaat gtacctcagc tgaaaccagt ttcggaattg acttttctca
     7141 aaagatcttt caatttggta gaggatagaa ttagacctgc aatttcggaa aaaacaattt
     7201 ggtctttaat agcatggcag agaagtaacg ctgagtttga gcagaattta gaaaatgctc
     7261 agtggtttgc ttttatgcat ggctatgagt tttatcagaa attttattat tttgttcagt
     7321 cctgtttgga gaaagagatg atagaataca gacttaaatc ttatgattgg tggagaatga
     7381 gattttatga ccagtgtttc atttgtgacc tttcatgatt tgtttaaaca aattttctta
     7441 aaatttctga ggtttgttta tttcttttat cagtaaat
//
